Directed Electronics equalizer 2500 Betriebsanweisung PDF herunterladen (Seite 104)

Perform a Rapid Run

Part # 15035786 Rev. D

Figure 47 Flow Cell Lever in Position 2

5 Make sure that the Vacuum Engaged checkbox is selected on the load sequencing flow

cell screen.

Confirm Proper Flow

1 Select solution 5 (USB) from the drop-down list.

2 Make sure that the following default values are entered:

• Volume: 250

• Aspirate Rate: 1500

• Dispense Rate: 2000

3 Make sure that waste outlet tubes 1, 2, 3, 6, 7, and 8 are in a bottle of clean water and

that tubes 4 and 5 are in the waste container.

4 Select Pump.

5 Visually inspect the flow cell for bubbles passing through the lanes and leaks near the

manifolds.

If excessive bubbles are present, check the manifold gaskets for obstructions, and repeat

the process.

a Select solution 6 (USB) to avoid depleting USB from position 5.

b Reduce the aspirate rate to 1000, and pump another 250 µl of USB to the flow cell.

6 After you have confirmed proper flow, select Next to proceed.

7 Make sure that the flow cell lever is green, and then close the flow cell compartment

door.

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Directed Electronics equalizer 2500 Betriebsanweisung Seite 104