Directed Electronics equalizer 2500 Betriebsanweisung PDF herunterladen (Seite 17)

HiSeq 2500 Software

HiSeq 2500 System User Guide

HiSeq 2500 Software

Three software applications are installed on the instrument computer:

} HiSeq 2500 control software—The HiSeq Control Software (HCS) interface guides you

through the steps to set up a sequencing run. During the run, the control software

operates instrument hardware, controls fluidics, sets temperatures, and provides a

visual summary of quality statistics.

} Real-Time Analysis software—Integrated with the control software, Real-Time

Analysis (RTA) performs base calling and assigns a quality score to each base for each

cycle. For more information, see Real-Time Analysis on page 111.

} Sequencing Analysis Viewer software—Sequencing Analysis Viewer (SAV) provides

detailed quality statistics.

HiSeq 2500 Control Software Interface

The Mode Select screen provides run mode options. Modes include TruSeq v3, HiSeq v4,

and Rapid Run. Select a run mode to proceed to the Welcome screen. Because only runs of

the same mode can be performed simultaneously, the selected mode is applied to flow cell

A and flow cell B.

The Welcome screen is split into 2 panels, 1 for each flow cell. You can set up a run for

flow cell A and flow cell B in parallel using the software interface. Runs can also be set up

independently using the software interface.

The Welcome screen provides commands to begin a sequencing run, wash the instrument,

perform a system check, and change modes. The current mode appears at the top of the

screen. When a run is complete, the software prompts to wash the instrument. After the

wash, the software returns to the Welcome screen.

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Directed Electronics equalizer 2500 Betriebsanweisung Seite 17