
Prepare Reagents for Read 2
HiSeq 2500 System User Guide
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Prepare Reagents for Read 2
Before the completion of Read 1 and any index reads, prepare reagents for Read 2
resynthesis and fresh ICB for Read 2.
For reagent preparation instructions, see the TruSeq PE Cluster Kit v3 Reagent Prep Guide
(part # 15023336). This guide includes instructions for preparing sequencing reagents
provided in the TruSeq Dual Index Sequencing Primer Box.
NOTE
For optimal performance, Illumina recommends preparing fresh ICB (Incorporation Mix) for
Read 2.
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