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Load and Prime Reagents

HiSeq 2500 System User Guide

Figure 36 Loading Station

NOTE

The liquid remaining in the tube after the run is highly diluted and not suitable for

further use.

d Slowly close the loading station door, making sure that the sippers are properly

aligned with the Eppendorf tubes when the lid is closed.

3 Select the Template loaded and template loading station closed checkbox.

4 Select Next.

Prime Reagents

NOTE

Prime reagents only if the HiSeq or TruSeq Rapid Duo Sample Loading Kit was used to

perform template hybridization on the cBot. Otherwise, skip reagent priming and proceed to

Load a Flow Cell on page 90.

Steps for priming reagents include cleaning the flow cell holder, loading a used flow cell,

confirming proper flow, and then starting the prime.

Clean the Flow Cell Holder

1 Open the flow cell compartment door.

CAUTION

Do not place fluids on the flow cell compartment door or on the flow cell stage when the

door is open. Spills in this area can damage the instrument.

2 Make sure that the flow cell lever is in the OFF position.

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Directed Electronics equalizer 2500 Betriebsanweisung Seite 95