Directed Electronics equalizer 2500 Betriebsanweisung PDF herunterladen (Seite 59)

Enter Run Parameters

HiSeq 2500 System User Guide

a SBS: TruSeq SBS Kit v3

b Index: TruSeq Multiplex Sequencing Primer Box or TruSeq Dual Index

Sequencing Primer Box

c PE turnaround: TruSeq PE Cluster Kit v3

5 [Optional] Select the Use Existing Recipe to use a custom recipe. Otherwise, allow the

software to create the recipe from run parameters entered.

Sample Sheet Screen

Sample sheets are optional unless you use BaseSpace to perform data analysis or perform

an indexed run.

1 Select Browse to navigate to the sample sheet location.

2 Select Next.

NOTE

HiSeq Control Software v2.2 allows a different indexing scheme in each lane.

Reagents Screen

The Reagents screen records information about reagent kits used for the run. The reagent kit

ID (barcode number beginning with RGT) is used to determine reagent kit type and run

mode compatibility.

1 Scan or enter the SBS reagent kit ID.

2 For paired-end runs, scan or enter the reagent kit ID for paired-end cluster kit.

3 Select the SBS reagent kit for the run:

• Select 200 Cycles for a 200 cycle kit. Cycles remaining defaults to 209.

• Select 50 Cycles for a 50 cycle kit. Cycles remaining defaults to 59.

• Select Custom for a partial kit or multiple 50-cycle kits. In the Cycles Remaining

field, enter the number of SBS cycles that reagents are expected to last.

NOTE

For partial kits, the software counts down the number of cycles entered. When the

cycles are low, the software prompts you to load fresh reagents.

4 Select Prime SBS Reagents to prime reagents before starting a run. Always prime

reagents before loading a new flow cell.

5 Select Next.

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Directed Electronics equalizer 2500 Betriebsanweisung Seite 59