Directed Electronics equalizer 2500 Betriebsanweisung PDF herunterladen (Seite 13)

HiSeq 2500 Components

HiSeq 2500 System User Guide

HiSeq 2500 Components

The HiSeq 2500 system comprises the instrument, monitor, instrument control computer,

and accessories, such as a keyboard, mouse, and barcode scanner. The instrument includes

4 main compartments: the optics module, flow cell compartment, fluidics compartment,

and reagents compartment. Instrument operating status is indicated on an illuminated

status bar.

Figure 1 External Components

A Optics module—Contains optical components that enable dual surface imaging of the

flow cell, imaging A, C, G, and T at the same time using epifluorescence. The excitation

laser beam passes through the objective and the fluorescence is collected through the

same objective.

B Flow cell compartment and library loading station—Contains the vacuum-controlled

flow cell stage, which holds the flow cell in place during sequencing runs. Using Rapid

Run mode, the loading station transfers libraries to the flow cell for on-instrument

cluster generation.

C Fluidics compartment—Contains fluidics pumps that deliver reagents to the flow cell,

and then to the waste container.

D Status bar—Uses 3 colors to indicate instrument status. Blue indicates that the

instrument is running, orange indicates that the instrument needs attention, and green

indicates that the instrument is ready to begin the next run.

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Directed Electronics equalizer 2500 Betriebsanweisung Seite 13