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HiSeq 2500 Software

HiSeq 2500 System User Guide

} Wash—Select Wash to initiate an instrument water wash or maintenance wash.

Figure 6 Wash Command Options

• Water Wash—The water wash flushes water through the system. This wash is

required after a sequencing run or after the instrument has been idle for 1 day or

more. See Perform a Water Wash on page 104.

• Maintenance Wash—The maintenance wash flushes Tween 20 and ProClin 300

through the system. This wash is required before switching modes or every 10

days, and is a recommended option after a high output run. See Perform a

Maintenance Wash on page 100.

} Check—Select Check to open the fluidics check screen and confirm proper flow during

instrument installation or fluidics troubleshooting.

} Mode Select—Select Mode Select to change run modes. Run modes include TruSeq v3,

HiSeq v4, and RapidRun.

Activity and Sensor Indicators

The Welcome screen contains a series of icons in the lower-right corner of the screen that

indicate instrument activity and status of specific components based on instrument

sensors.

Figure 7 Activity Indicators

From left to right, activity indicators represent the X, Y, and Z motors, electronics

functionality, the camera, the fluidics system, and processing functions.

Figure 8 Sensor Indicators

From left to right, sensor indicators represent flow cell A temperature, reagent chiller

temperature, BaseSpace cloud status, and flow cell B temperature.

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Directed Electronics equalizer 2500 Betriebsanweisung Seite 19