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Load and Prime Reagents

HiSeq 2500 System User Guide

Load a Priming Flow Cell

NOTE

Use a used flow cell to prime reagents. Do not use the flow cell that you want to sequence.

1 Rinse a used flow cell with laboratory-grade water. Dry it with lens cleaning tissue or

lint-free tissue.

2 Clean the flow cell using alcohol wipes and lens cleaning tissue.

NOTE

Do not remove or replace the manifold gaskets.

3 Place the used flow cell on the flow cell holder with the inlet and outlet ports facing

down and the barcode on the right. Make sure that the arrow on the left edge of the

flow cell, which indicates flow direction, points towards the instrument.

4 Gently slide the flow cell towards the top and right guide pins until it stops.

Figure 39 Flow Cell Positioned Against Top and Right Guide Pins

A Top Guide Pin

B Right Guide Pins

NOTE

Remove your hand from the flow cell before engaging the vacuum switch to prevent

possible alignment drift over time.

5 Slowly move the flow cell lever to position 1 to engage the vacuum and secure the flow

cell into position. When the flow cell lever is green, the vacuum is engaged.

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Directed Electronics equalizer 2500 Betriebsanweisung Seite 97