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Perform a Rapid Run

Part # 15035786 Rev. D

Rapid Run Sequencing Workflow

Prepare all reagents for the run and prepare the library template.

For reagent preparation information, see Reagent Preparation Steps on page

70.

Using the control software, perform a volume check and enter run

parameters.

For on-instrument clustering—Load all reagents for the run and the

prepared library template.

For cluster generation on the cBot—Load all reagents for the run.

With a used flow cell on the instrument, confirm proper flow.

For cluster generation on the cBot—Prime SBS reagents and measure

priming waste.

Start the sequencing run. After cycle 1, inspect the first base report

(optional setting), and then continue Read 1.

The sequencing run continues through the PE turn and Read 2 with no

intervention necessary.

When the run is complete, unload and weigh reagents. Perform a post-run

water wash.

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Directed Electronics equalizer 2500 Betriebsanweisung Seite 82