Directed Electronics equalizer 2500 Betriebsanweisung PDF herunterladen (Seite 39)

Load and Prime Reagents

HiSeq 2500 System User Guide

Load Paired-End Reagents

1 Record the weight of each reagent on the lab tracking form.

2 Raise the sippers for the paired-end reagent rack using the following motion:

a Pull the handle towards you and then raise the handle.

b Release the handle into the slot on the top end of the groove. Make sure that the

handle rests securely in the slot.

3 Slide the reagent rack out of the reagent compartment using the rack handle.

4 Remove the caps from each reagent tube and place the tube onto the rack in the

associated numbered position or matching label color.

Position

Reagent Description

FRM * Fast Resynthesis Mix

FLM2 Fast Linearization Mix 2

AMS Fast Amplification Mix

FPM Fast Amplification Premix

FDR * Fast Denaturation Reagent (contains formamide)

HP11 Read 2 Sequencing Primer

Table 4 Paired-End Flow Cells

* If you loaded indexing reagents for a single-index run, FDR is already loaded in position 10. If you

loaded indexing reagents for a dual-index run, FRMand FDR are already loaded in positions 10 and

15, respectively.

5 Place 15 ml conical tubes filled with 10 ml laboratory-grade water in unused positions

on the paired-end rack.

6 Slide the reagent rack into the reagent compartment, aligning the rack with the raised

guide on the floor of the compartment.

7 Lower the sippers into the paired-end reagent tubes as follows:

a Pull the handle towards you and then lower the handle.

b Visually inspect the sippers to make sure that they do not bend as they lower into

the tubes.

c Release the handle into the slot on the bottom end of the groove.

8 Select Next.

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Directed Electronics equalizer 2500 Betriebsanweisung Seite 39