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Load and Prime Reagents

HiSeq 2500 System User Guide

Figure 11 Vacuum Hole Locations

Load a Priming Flow Cell

From the Load Priming Flow Cell screen, load a used flow cell for the priming step. After

loading a used flow cell, confirm that the vacuum is engaged.

NOTE

Illumina recommends using the flow cell from a previous run for priming reagents on a

subsequent run or for a post-run instrument wash.

1 Rinse the used flow cell with laboratory-grade water. Dry the flow cell with a lens

cleaning tissue or lint-free tissue.

2 Clean the flow cell using alcohol wipes and lens cleaning tissue.

NOTE

Do not remove or replace the flow cell gaskets during this step.

3 Place the used flow cell on the flow cell holder with the inlet and outlet ports facing

down and the barcode on the right. Make sure that the arrow on the left edge of the

flow cell, which indicates flow direction, points towards the instrument.

4 Gently slide the flow cell towards the top and right guide pins until it stops.

NOTE

Remove your hand from the flow cell before engaging the vacuum switch to prevent

possible alignment drift over time.

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Directed Electronics equalizer 2500 Betriebsanweisung Seite 41