Directed Electronics equalizer 2500 Betriebsanweisung PDF herunterladen (Seite 64)

Perform a TruSeq v3 Run

Part # 15035786 Rev. D

10 Select Next.

Prime Reagents

Steps for priming reagents include cleaning the flow cell holder, loading a priming flow

cell, confirming proper flow, and then starting the prime.

Clean the Flow Cell Holder

1 Open the flow cell compartment door.

CAUTION

Do not place fluids on the flow cell compartment door or on the flow cell stage when the

door is open. Spills in this area can damage the instrument.

2 Make sure that the flow cell lever is in the OFF position.

Figure 23 Flow Cell Lever in Position 0

3 Put on a new pair of powder-free latex gloves.

4 If the flow cell from a previous run is present, remove it and set aside in a tube of

storage buffer or laboratory-grade water to keep it from drying out. It can be used to

confirm proper flow before loading the clustered flow cell.

5 Using an alcohol wipe or a lint-free tissue moistened with ethanol or isopropanol,

carefully wipe the surface of the flow cell holder until it is clean.

CAUTION

Do not allow alcohol to drip into the vacuum holes or around the manifolds. Use a low-lint

lab tissue to dry the stage, if necessary.

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Directed Electronics equalizer 2500 Betriebsanweisung Seite 64