Directed Electronics equalizer 2500 Betriebsanweisung PDF herunterladen (Seite 77)

Load Reagents for Read 2

HiSeq 2500 System User Guide

7 Slide the reagent rack into the reagent compartment, aligning the rack with the raised

guide on the floor of the compartment.

8 Lower the sippers into the paired-end reagent tubes as follows:

a Pull the handle towards you and lower the handle.

b Visually inspect the sippers to make sure that they do not bend as they lower into

the tubes.

c Release the handle into the slot on the bottom end of the groove.

Load ICB for Read2

1 Record the weight of the reagent on the lab tracking form.

2 Raise the sippers for the sequencing reagent rack using the following motion:

a Pull the sipper handle towards you and raise the sipper handle.

b Release the sipper handle into the slot on top end of the groove. Make sure that the

sipper handle rests securely in the slot.

3 Slide the reagent rack out of the reagent compartment.

4 Remove the existing ICB reagent bottle from position1 of the reagent rack and remove

the funnel cap from the bottle.

5 Place the funnel cap on the new bottle of ICB and load the bottle in position1. Make

sure that the conical end of the bottle rests in the indentation on the base of the rack.

6 Slide the reagent rack into the reagent compartment, aligning the rack with the raised

guide on the floor of the compartment.

7 Lower the sippers into the sequencing reagent bottles as follows:

a Pull the sipper handle towards you and lower the sipper handle.

b Visually inspect the sippers to make sure that they do not bend as they lower into

the funnel caps.

c Release the sipper handle into the slot on the bottom end of the groove.

8 Close the reagent compartment door, and then select Next to resume the run.

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Directed Electronics equalizer 2500 Betriebsanweisung Seite 77