Directed Electronics equalizer 2500 Betriebsanweisung PDF herunterladen (Seite 49)

Load a Flow Cell

HiSeq 2500 System User Guide

Figure 21 Flow Cell Lever in Position 2

5 Make sure that the Vacuum Engaged checkbox is selected on the Load Sequencing

Flow Cell screen.

Confirm Proper Flow

Checking for proper flow confirms that the flow cell and gaskets are properly installed and

the manifold is engaged.

1 Select solution 5 from the drop-down list.

2 Enter the following default values:

• Volume: 250

• Aspirate Rate: 250

• Dispense Rate: 2000

3 Select Pump.

4 Visually inspect the flow cell for bubbles passing through the lanes or leaks near the

manifolds.

If excessive bubbles are present, check the manifold gaskets for obstructions and repeat

the process using solution 6 to avoid depleting position 5. Reduce the aspirate rate to

100, and pump another 250 µl to the flow cell.

5 Select Next. Make sure that the flow cell lever is green, and then close the flow cell

compartment door.

6 Confirm that the checkboxes Vacuum Engaged and Door Closed are selected, and then

select Next.

7 Select Start to start the sequencing run.

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Directed Electronics equalizer 2500 Betriebsanweisung Seite 49