Directed Electronics equalizer 2500 Betriebsanweisung PDF herunterladen (Seite 116)

Post-Run Procedures

106

Part # 15035786 Rev. D

Switch Sequencing Modes

Use the Mode Select command from the Welcome screen to switch between high output

modes and Rapid Run mode.

Only runs of the same mode can be performed simultaneously. Therefore, mode changes

are applied to both flow cell A and flow cell B. If either flow cell is in progress, a mode

change is not possible.

A maintenance wash and gasket change are required when switching between run modes.

For more information, see Perform a Maintenance Wash on page 100.

Switch from High Output to Rapid Run Mode

Switching from a high output mode (HiSeq v4 or TruSeq v3) to Rapid Run mode requires a

rapid-mode maintenance wash.

Specification Rapid Maintenance Wash

Flow Cell Type

Rapid flow cell (2 lanes)

Flow Cell Gasket

10-port gasket and 8-port gasket

Reagents

Tween 20 and ProClin 300

Expected Volumes (ml)

60.2 ml

Time (minutes)

60 minutes

Switch from Rapid Run to High Output Mode

Switching from Rapid Run mode to a high output mode (HiSeq v4 or TruSeq v3) requires a

rapid-mode maintenance wash followed by high-output maintenance wash.

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Directed Electronics equalizer 2500 Betriebsanweisung Seite 116