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Troubleshooting

134

Part # 15035786 Rev. D

• Keep As Is—The run is stopped without any modifications to Real-Time Analysis.

The run can resume where it was stopped.

• Complete For Run—Real-Time Analysis is stopped. The run info, run parameters,

and recipe files are updated to reflect total cycles as the last cycle completed. Then

Real-Time Analysis restarts to complete base calling for the run up to the point the

run was stopped. The run cannot resume.

• Complete For Read—Real-Time Analysis is stopped. The run info, run parameters,

and recipe files are updated to trim the length of the current read to the last cycle

completed. Subsequent reads are not affected. Then Real-Time Analysis restarts to

complete analysis for the current read. The run can resume at the beginning of the

next read.

5 After the run is stopped, select Return to Start on the Run Overview screen. The

Welcome screen opens.

Resume a Stopped Run

A run can be resumed only when the run was stopped safely using a normal stop option

with a Real-Time Analysis option that allows the run to resume.

NOTE

If the adjacent side is performing cluster generation or paired-end chemistry, the run does

not resume until the ongoing process is complete.

1 From the Welcome screen, select Sequence, and then select Resume Run. The Resume

screen opens.

2 Select the appropriate run folder from the drop-down list.

NOTE

The software resumes the run at the point where the run was stopped, and defaults to the

correct setup on the Resume screen.

3 Confirm the settings on the Resume screen or select the appropriate point in the run to

resume the run.

• Resume At lists the read or point in the run to resume.

• Start At Cycle lists the cycle to resume.

CAUTION

Illumina does not recommend resuming a run at the point of paired-end turnaround, other

than for Read 2 primer rehybridization.

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Directed Electronics equalizer 2500 Betriebsanweisung Seite 144