Directed Electronics equalizer 2500 Betriebsanweisung PDF herunterladen (Seite 12)

Overview

Part # 15035786 Rev. D

Introduction

The HiSeq

system combines innovative engineering with proven SBS technology to set

new standards in output, simplicity, and cost-effectiveness.

The HiSeq 2500 includes the following features:

} Dual-surface imaging—The HiSeq 2500 uses a 4-camera epifluorescence system with

cutting-edge scanning technology to enable dual surface imaging.

} Dual flow cells—The HiSeq 2500 is a dual flow cell system, which allows sequencing

of a single flow cell or 2 flow cells with different read lengths simultaneously.

} On-instrument cluster generation—The HiSeq 2500 provides the option of Rapid Run

mode, which includes on-instrument cluster generation.

} High-capacity reagent chiller—The reagent compartment is a high-capacity chiller that

holds enough reagents for the entire sequencing run.

} Integrated fluidics for paired-end runs—Integrated paired-end fluidics provide

reagents from the reagent compartment to the flow cell for Read 2 resynthesis and for

indexed sequencing.

} Interface control options—The instrument software interface provides options for

setting up a run and operating the instrument using the touch screen monitor or the

integrated keyboard.

} Real-time base calling—The instrument software extracts intensities from images and

performs quality-scored base calling on the instrument computer, which allows

monitoring of quality metrics during the run and saves time during subsequent data

analysis.

Downstream analysis of sequencing data can be performed with Illumina analysis

software or third-party software on IlluminaCompute, Illumina BaseSpace, or a custom

infrastructure.

} BaseSpace connectivity—The HiSeq 2500 features an option to send instrument health

and sequencing data to the BaseSpace genomics cloud solution in real time to

streamline instrument quality control and analysis.

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Directed Electronics equalizer 2500 Betriebsanweisung Seite 12