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Directed Electronics equalizer 2500 Betriebsanweisung Seite 93

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Load and Prime Reagents
HiSeq 2500 System User Guide
83
9 Select the PW1 (25 ml) loaded checkbox.
10 Slide the PEreagent rack out of the reagent compartment.
11 Remove the caps from each reagent tube and place the tube onto the rack in the
associated numbered position.
Position Reagent Description
10 PW1
10 ml PW1 or laboratory-grade water
11 PW1
10 ml PW1 or laboratory-grade water
12 PW1
10 ml PW1 or laboratory-grade water
13 AMS
Fast Amplification Mix
14 FPM
Fast Premix
15 FDR
Fast Denaturation Reagent (contains formamide)
16 HP9*
i5 Index Primer
17 HP12*
i7 Index Primer
18 HP10
Read 1 Primer
19 FLS
Fast Linearization Solution
Table 14 Single-Read Flow Cell
*HP9 is required only for dual-indexed runs. HP12 is required for all indexing options. If HP9 and
HP12 are not used, load a 15 ml conical tube filled with 10 ml PW1 or laboratory-grade water in
position 16 for HP9 and position 17 for HP12.
Position Reagent Description
10 FRM
Fast Resynthesis Mix
11 FLM2
Fast Linearization Mix 2 (Read 2)
12 FLM1
Fast Linearization Mix 1 (Read 1)
13 AMS
Fast Amplification Mix
14 FPM
Fast Premix
15 FDR
Fast Denaturation Reagent (contains formamide)
16 HP11
Read 2 Primer
17 HP12*
i7 Index Primer
18 HP10
Read 1 Primer
19 PW1
10 ml PW1 or laboratory-grade water
Table 15 Paired-End Flow Cell
*HP12 is required only for indexed runs. If HP12 is not used, load a 15 ml conical tube filled with
10 ml PW1 or laboratory-grade water in position 17.
12 Add 10 ml PW1 or laboratory-grade water to 15 ml conical tubes in the following
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