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Perform a TruSeq v3 Run

Part # 15035786 Rev. D

Introduction

To perform a TruSeq v3 run on the HiSeq 2500, prepare SBS reagents for Read 1 and

indexing reagents before setting up the run. Follow the software prompts to set up the run,

which includes entering run parameters, loading and priming reagents, loading the flow

cell, and performing a fluidics check.

Prepare and load paired-end reagents and SBS reagents for Read 2 after the completion of

Read 1 and any index reads.

Visit the HiSeq 2500 specifications page on the Illumina website for information about run

duration and other performance specifications.

TruSeq v3 Sequencing Consumables

TruSeq v3 Kit Name Description

TruSeq SBS Kit v3 (200 Cycles)

TruSeq SBSKit v3 (50 Cycles)

Contains SBSreagents used on the HiSeq 2500.

TruSeq PE Cluster Kit v3

TruSeq SRCluster Kit v3

Contains clustering reagents used on the cBot and indexing

reagents used on the HiSeq 2500.

The PE version of the cluster kit includes paired-end reagents

used on the HiSeq 2500.

Each cluster kit includes an accessories kit that contains flow cell

gasket replacements and funnel caps for SBSreagent bottles.

Reagent Preparation Steps

} For SBS reagent preparation, see the appropriate guide:

• TruSeq SBS Kit v3 Reagent Prep Guide (200 Cycles) (part # 15023333)

• TruSeq SBS Kit v3 Reagent Prep Guide (50 Cycles) (part # 15023334)

} For indexing and paired-end reagent preparation, see the appropriate guide:

• TruSeq PE Cluster Kit v3 Reagent Prep Guide (part # 15023336)

• TruSeq SR Cluster Kit v3 Reagent Prep Guide (part # 15023335)

These guides include instructions for preparing sequencing primers provided in the

TruSeq Dual Index Sequencing Primer Box.

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Directed Electronics equalizer 2500 Betriebsanweisung Seite 52