Directed Electronics equalizer 2500 Betriebsanweisung PDF herunterladen (Seite 88)

Perform a Rapid Run

Part # 15035786 Rev. D

A first base report is generated automatically for each run. Selecting this option opens

the first base report before proceeding with the run.

2 [Optional] From the Align to PhiX checkboxes, clear the checkbox for lanes that do not

contain PhiX.

By default, all lanes are selected for alignment by Real-Time Analysis.

Alternatively, select lanes on the flow cell image to add or remove lanes for PhiX

alignment.

NOTE

A dedicated control lane is not required with HCS v2.2 and RTA v1.18. Therefore, the

option to assign a control lane is not available with this software configuration.

3 [Optional][For TruSeq Rapid v1] Select Keep Intensity Files for later reanalysis or

custom processing.

By default, this option is not selected. Saving intensity files is not required for on-

instrument analysis. Enabling this option significantly increases the size of the data

output folder.

4 Select Next.

Recipe Screen

A recipe is generated automatically from the information entered on the Recipe screen.

1 Select from the following Index Type options:

• No Index—Performs a non-indexed single-read or paired-end run.

• Single Index—Performs a single-read or paired-end run with 1 indexing read.

• Dual Index—Performs a single-read or paired-end run with 2 indexing reads.

• Custom—Performs a single-read or paired-end run with a custom number of cycles

for index reads.

2 If the Dual Index or Custom option is specified, select a Flow Cell Format, either Single

Read or Paired End.

3 Enter the number of cycles for Read 1 and Read 2, if applicable.

NOTE

The number of cycles performed in a read is 1 more cycle than the number of cycles

analyzed. For example, to perform 100 cycles for Read 1, enter 101.

For the Custom indexing option, enter the number of cycles for index reads. Read

lengths do not need to be identical.

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Directed Electronics equalizer 2500 Betriebsanweisung Seite 88